Smita Purohit
The IIS University, India
Title: Influencing regeneration potential in Dianthus caryophyllus L. (carnation) by manipulation of culture medium
Biography
Biography: Smita Purohit
Abstract
Growth and morphogenesis of plant tissues under in vitro conditions are largely influenced by the composition of the culture media. Therefore, in this study, effects of copper sulphate in regeneration medium of Dianthus caryophyllus were examined. Nodal segments were cultured on MS medium supplemented with BAP (0.5 mg l-1) + NAA (0.5 mg l-1) and different levels of CuSO4 (0, 0.1*, 1, 2, 3, 5, 10 µM). The levels of CuSO4 in the induction as well as proliferation medium highly influenced the shoot regeneration. Highest number of shoot buds per explant was obtained when the concentration of CuSO4 was increased two times the normal MS level. The effect of various antioxidant enzyme activities (catalase, SOD and peroxidase) was studied on different levels of copper sulphate. It was found that the enzyme activities increased with the increasing levels of copper sulphate and also increased with increased morphogenic competence of the in vitro cultures. The enzyme activities showed a decline where the number of shoot buds was less. Therefore, this confirms a relationship between the organogenesis and stress levels in the in vitro cultures.