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Smita Purohit

Smita Purohit

The IIS University, India

Title: Effect of vitamins on improving morphogenic competence in Cuminum cyminum L. cultures


Biography: Smita Purohit


Cuminum cyminum L. is an important seed spice belonging to family Apiaceae. Production of cumin is limited due to limited genetic diversity and several biotic stresses. Hence, the present study aims at improving the regeneration of cumin in in vitro cultures. A regeneration medium was standardized comprising of MS supplemented with kinetin (0.5 mg/l). Effect of thiamine was studied on the morphogenic competence of the in vitro cultures. The levels of thiamine in the induction as well as proliferation medium highly influenced the shoot regeneration. Highest number of shoot buds per explant was obtained when the concentration of thiamine was twofold the normal MS level at both induction as well as proliferation stages. Shoots upto 2 cm or more in length were excised and inoculated on rooting medium i.e., MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA). Rooted plantlets were transferred to field conditions.