Dina Atmani-Kilani
University of Bejaia, Algeria
Title: Anti-ulcer activities of a local plant from Algeria, Clematis fl ammul
Biography
Biography: Dina Atmani-Kilani
Abstract
Statement of the Problem: Gastric ulcer is a widely distributed disease characterized by lesions in the gastric mucosa leading to inflammatory condition and an increase in oxidative stress. Clematis flammula (Ranunculaceae) is a medicinal plant largely used by rural populations in Algeria to treat inflammatory disorders like rheumatoid arthritis. However, its use against gastric ulcer is limited. The purpose of this study was to examine the gastroprotective effect of the leaves ethanolic extract of C. flammula in an animal model.
Methodology & Theoretical Orientation: Gastric ulcer was induced by indomethacin (25mg/kg) in male mice, one hour after the administration of ethanolic extracts of C. flammula at different concentrations (25, 50, 100 and 200mg/kg) and the reference drugs misoprostol Omeprazole and Bromazepam. The anti-ulcer activity of the extracts was evaluated by the estimation of the ulcer score (UC), the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione and MDA levels in the tissue homogenates of the stomach. Histological analysis boosted by the extract, mainly at 100 mg/kg from 16.68±0.96µmoles/mg/min in the indomethacin-treated group to 28.99±3.2 comparable to misoprostole (26.4±2.49µmoles/mg/min) administered at 200µg/Kg misoprostol. The depletion of glutathione level by indomethacin (2.76±0.63 μmoles/mg prot) in the ulcerated group was prevented by the pre-treatment with 100mg/kg of Clematis flammula leaves extract and was restored to a normal level (30.95 ± 2.76 μmoles/mg prot). Histological analysis confirmed the results.
Conclusion & Significance: These results prove that the antioxidant activity of the plant contributes to its gastroprotective potential was also performed to confirm the results