Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 3rd Global Summit on Plant Science Rome, Italy.

Day 2 :

Keynote Forum

Leon D van Rensburg

University of the Free State, South Africa

Keynote: Inferring salt and water distribution in an irrigated crop field using electromagnetic induction techniques

Time : 09:00-09:30

Conference Series Plant Science 2017 International Conference Keynote Speaker Leon D van Rensburg photo
Biography:

Leon Daniel van Rensburg is a soil physicist lecturing soil and water management at the University of the Free State, South Africa. He established and maintained a vibrate post-graduate research team, which currently consisted of 2 post-doctoral fellows, 4 doctoral students and 8 master students. The team focus mainly on soil and water management off dryland, irrigation as well as natural field and they published in the last 6 years about 45 scientific articles. He is leading two projects of national importance for South Africa, viz. (i) Management guidelines for technology transfer to reduce salinisation of irrigated land with precision agriculture funded by the Water Research Commission and (ii) the soil water balance of the Ghaap Platue as influenced by open cast mining funded by Sishen Iron Ore Company (Anglo American Kolomela). Both projects are scheduled for five years.

Abstract:

Statement of the Problem: Irrigation through its addition of salts poses a continuous threat to the functioning of natural resources. Estimates indicated that of the total land being irrigated in South Africa, 1-12% is severely waterlogged or salt affected, and 5-20% moderately affected. The affected areas need to be reduced through sound water management. Fortunately, recent advances in EMI technology opened new opportunities for site-specific crop management. The objectives of this study were (i) to evaluate the reliability of the EM38-MK2 to infer soil properties, like particle sizes, electrical conductivity (EC), sodium adsorption ratio (SAR) and soil water, and (ii) to delineate salt affected zones. Methodology & Theoretical Orientation: The 56 ha crop field is located on the banks of the Vaal River in the Northern Cape Province, South Africa. The long-term water quality of the river is good (EC of 52 mS m¹ and sodium adsorption ratio of 1.2). The soils of the field vary from sandy to clay. The field was scanned with an EM38-MK2 during September 2016 to a depth of 1.5 m with inter-row spacing of 20 m. Twelve soil profiles were sampled to a depth of 1.5 m over 0.3 m increments. The soils were analyzed for soil water, particle sizes and the saturated paste was used for measuring EC, cations (to calculate SAR) and pH. These values were then correlated with the apparent EC recorded by the EM38-MK2. Findings: Results showed that all the soil properties, except pH, correlated significantly with the measured ECa. Spatial maps for the properties were drawn and the salt affected areas delineated. Conclusion & Significance: The research demonstrated that the EM38-MK2 can be used to infer the spatial distribution of salts in crop fields, which is a critical step in the amelioration proses.

Conference Series Plant Science 2017 International Conference Keynote Speaker Sergey Dolgov photo
Biography:

Sergey Dolgov is the Head of Laboratory of Expression Systems and Plant Genome Modifi cation “Biotron”. During last 25 years, the technologies of in vitro cultivation of isolated cells, tissues and organs on artifi cial media have been developed for more than plant 30 species. Highly effective methods of genetic transformation have been developed for a large number of plants (carrots, tomatoes, pears, apples, strawberries, wheat, duckweed, chrysanthemum), which allow to study the activity of foreign proteins in transgenic plants and obtain varieties with economically valuable traits. Currently, the station of artifi cial climate "Biotron" researches on the plant physiology and molecular biology (studying of genes that affect the fl owering morphology of Compositae), biopharming, protection of plants against biotic and abiotic stresses, fi eld
trials of transgenic fruit trees, etc.

Abstract:

Plum pox virus (PPV) is the serious viral disease affecting Prunus species such as plum, apricot, cherry and peach. To date the few PPV resistance genetic resources found in Prunus germplasm, nevertheless the conventional breeding approaches are very challenging for use in fruit trees due to several limiting intrinsic factors. Methodology & Theoretical Orientation: Several biotechnological approaches could be used to develop PPV resistance in plants; nonetheless, the RNA interference is shown to be the most effective disease-control strategy (Ilardi and Tavazza 2015). In our initial report we have successfully used this      biotechnological technology to produce transgenic plants of commercial cultivar “Startovaja” (Prunus domestica L.) with PPV-derived ihpRNA construct (Mikhailov and Dolgov 2011). The transformation experiments were conducted using genetic construct containing the self-complementary sequences of fragment of PPVCP gene separated by an intron for the induction Plum Pox Virus (PPV) resistance through the mechanism of post-transcriptional gene silencing. Transgenic plum rootstocks plants have been produced from organogenic callus developed on leaf explants within 6-month culture after the inoculation. Conclusion & Significance: PCR-analysis confirmed the transgenic status of produced plants by the amplification of the fragments of “hairpin”-PPV-CP construct and hpt gene. To our knowledge, this is the first report of the successful attempt to produce transgenic plum rootstock

Break: WORKSHOP